. The SpectraMax M5 Multi-Mode Microplate Reader measures UV and visible absorbance, fluorescence intensity, glow luminescence, fluorescence polarization and time-resolved fluorescence (TRF). 1) EarlyTox NucView 488 Assay (6. Multimode Readers Comparison Chart. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. Devices PathCheck® Sensor—the only patented technology available that measures the depth (optical pathlength) of samples in a microplate. SpectraMax 190: SpectraMax 340PC384: SpectraMax Plus384: VERSAmax: Vmax: Tecan: Sunrise: Trinean: DropSense96: AMBIENT TEMPERATURE STORAGE: Liconic: LPX 220 Hotel: LPX 440 Hotel: Thermo Fisher Scientific: Cytomat 10 Hotel: Cytomat 2 Hotel: Cytomat 24 Hotel: Cytomat 8 Linear Hotel: Cytomat 9 Hotel (MPH) BAR CODE. 1. 7°C and the bottom to 36. IP1 labeled with d2 acceptor competes with native (unlabeled) IP1 for binding to the donor-labeled IP1-specific monoclonal antibody. Open the SoftwarePro® Data Acquisition & Analysis software,. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. SpectraMax Glo and DuoLuc. The SpectraMax ® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of. There were 12 replicate wells per dilution in both 96-well and 384- well plates. Both the SpectraMax M5 and the Gemini EM are monochromator-based, microplate readers. 1). The purpose of the next experiment was to demonstrate that the Dual-Glo assay system could be used to measure the induction of pCREluc by forskolin. Microplate Readers Molecular Devices Spectramax M5 Microplate Reader. 190-1000 nm, tunable in 1 nm increments. spr SpectraMax M2 M2e M3 M4 M5 M5e SpectraTest ABS2 v2. . The SpectraMax® Gemini™ XPS Microplate Spectrofluorometer from Molecular Devices® provides a flexible environment to determine the optimal excitation and emission settings for most fluorescent intensity assays. Access this by going to Help >. . With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax ® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. The integration time suggested is 5-10 sec. Five preferred detection modes. HEALTH AND SAFETY CONSIDERAIONS StakMax Handling Stacker Base Unit. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. In this. Our absorbance plate readers feature our PathCheck Sensor technology and. 0 mm wide, and heights from 14 mm to 15 mm. 6. 4. Published Dec 16, 2020 | Updated Dec 16, 2020. 4. 0 out of 5 $ 5,999. 00 USD. The drawing shows a standard microplate of 15 mm height covered with a lid. . Download PDF versions of the SpectraMax M5 User Guides from this page. 3. Italic and boldface type are used for emphasis. Table of Contents. 40266 Email: drugdiscoverytech@lifetech. . 사용이 편리하고 견고한 생명과학 연구, 신약 개발, 개발, Bioassay용 assay. Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. acquired on a SpectraMax M5 (Molecular Devices). Molecular Devices recommends that you use high-quality, doubleshielded cables to connect your SpectraMax. Multimode Reader Comparison Chart. Do NOT use the front panel Ref button in fluorescence mode cuvette reads. Formula accessors for reporting o The accessor!sectionname will return the name of the SoftMax Pro section containing the. Set up template in SoftMax Pro and read plate on the SpectraMax M5 microplate reader: Note: IMAP FP protocols for use with FAM- and TAMRA-labeled substrates are available in SoftMax Pro 5 in the Binding Assays protocol folder. The PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. 7°C and the bottom to 36. The REF button has no valid meaning for. The PathCheck sensor can extend the instrument linearity by a factor of three or more. 0 (GraphPad Software, La Jolla, CA, USA) and SPSS version 23. Molecular Devices SpectraMax M5e User Manual (86 pages) Multi-Mode Microplate Readers. How do I do this? Note: Check the Applies to section at the bottom of the page to make this is the. When the user applies a set temperature of 37°C, the top is heated to 37. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. 00 USD. Certified SpectraMax® M5e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays’ HTRF (Homogeneous Time-Resolved Fluorescence) technology. Gemini XPS and EM Microplate Readers with dual monochromator fluorometers provide a flexible environment to determine the optimal excitation and emission settings for fluorescence intensity assays. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. 0 out of 5 $ 15,999. The M5 can read volumes down to 2uL with the. The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplates. As per Volza’s India Import data, Spectramax import shipments in India stood at 365, imported by 145 India Importers from 28 Suppliers. 72 MB. EarlyTox Live Cell Assay (6. . 2. 5. The assay applies fluorescence polarization to quantify Fc-containing IgG. With only a few product lines that we provide service for, our engineers have unrivaled expertise in the SpectraMax® microplate readers. Sensitive fluorescent quantitation of DNA with the Quant-iT PicoGreen dsDNA Assay Kit The Spectramax M5 has similar detection capability to the Analyst GT and LMax II 384 (Figure 4), so we are confident that its performance is also similar when measuring the enzymes in cell lysates. Description 1. SpectraMax® Microplate Readers and SoftMax® Pro Software. For example, microplates containing different samp les can be read using the same orPlates are used to validate optical performance of SpectraMax M3, M4, and M5/M5e readers. SpectraMax M2 and M2e Performance Specifications SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide — 0112-0102 Rev. A plate containing two columns each of 75 µL, 100 µL, 150 µL, 200 µL, 250 µL, or 300 µL of yellow buffer solution was read at 426 nm on a SpectraMax microplate reader. Description. Molecular Devices SpectraMax M5/M5e Microplate Reader was tested for compatibility with Life Technologies LanthaScreen® Kinase Binding and Adapta™ Europium-based TR-FRET assays. . Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. The SpectraMax® M5e Multi-Mode Microplate Reader delivers single-mode reader performance in one multi-mode reader package. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader. Highly selective for dsDNA over ssDNA or RNA. 5. My experiments primarily involve monitoring the change in tryptophan fluorescence with time, using 96. SoftMax Pro can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. Open the Softmax Pro software and select the FP option. Table of Contents. . This assay kit is optimized for the SpectraMax i3x Multimode Microplate Reader with a preconfigured protocol in SoftMax Pro Software for rapid. item 6 replacement bulb for molecular devices spectramax m5 xenon replacement bulb for molecular devices spectramax m5 xenon. ® reader to the computer. Spectramax M5 plate reader (96/384 format) Multimode photometrics for endpoint or kinetic measurements. 1. The absorbance and photo-luminescence was determined on a SpectraMax M5 plate reader via quartz cuvette using 1 × 10 10 particles per ml or lower and extrapolated when an optical density of 1 was. New drivers take 6 days to write, existing drivers take 2 days to convert to Windows 7/10 compatibility. Absorbance: For wavelengths in the visible range, 340 nm and above, clear polystyrene plates are recommended. Step 2. SoftMax Pro Software can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. 304 signal level saturation. Wavelength optimization. HEALTH AND SAFETY CONSIDERAIONS The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies GeneBLAzer® assays. edu) to be added as a trained user in iLab. Optical absorbance was measured at 570 nm on a SpectraMax M5 microplate reader. Example: [Drawer]. SpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Cuvette Chamber The cuvette chamber is located to the right of the control panel. 303 unable to cal dark current. 5. This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. 5. item 3 replacement bulb for molecular devices spectramax xps xenon, spectramax xs xenon replacement bulb for molecular devices spectramax xps xenon, spectramax xs xenon. 40266 Email: drugdiscoverytech@lifetech. Description 2 SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers Operator’s Manual — 0112-0115 Rev. INTRODUCTION The SpectraMax M2 and SpectraMax M2 e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as well SpectraMax M5 Multi-Mode Microplate Reader. Fix: While proper use of the Shake button is to hold the button down for the duration of the desired mixing time, repeated sequential clicking is now allowed. The chamber contains springs that position the cuvette in the proper alignment for a read. SpectraMaxi3,M3,M4,M5,M5e,andFlexStation 3instruments G03 through G08forSpectraMaxLinstrument Cross-talk D09 through D11,E09 through E11,andF09 through F11 2 SpectraMax MiniMax300ImagingCytometer(cytometer) 3. Cavicide (Warehouse, Cat # 79300360) 6. MDS Analytical Technologies announced the launch of the Molecular Devices® SpectraMax® M3 and M4 Multi-Mode Microplate Readers. 9000-0756. We would like to show you a description here but the site won’t allow us. . LUMINESCENCE The SpectraMax M2 and SpectraMax M2e are microplate spectrofluorometers with photomultiplier tube detection. ScanLater Western Blot Detection Cartridge. The BenchCel Microplate Handling Workstation provides microplate-orientation sensing so all microplates enter the reader in the same orientation, ensuring that the data output is consistent from the first microplate to the last. SpectraMax M5 and the Analyst GT. Add to Cart. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M5e Microplate Reader transforms each well in a microplate to a fixed. The Molecular Devices SpectraMax M5E is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. SoftMax Pro. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. Sale. . . The following document is intended to demonstrate setup of this instrument. SpectraMax Spectrofluorometers. Exhibit with us {{>currencyLabel}} Back. Twelve different automated tests are provided: • Fluorescence lower limit of detection (LLD) • Excitation wavelength accuracy • Emission wavelength accuracy • Excitation wavelength precision Here, we present FluoroBrite™ DMEM, a cell culture media designed specifically for live-cell imaging that, when excited at wavelengths commonly used for FITC/GFP (i. SoftMax Pro Software can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. spr SpectraMax M2 M2e M3 M4 M5 M5e SpectraTest ABS2 v1. Click SoftMax Pro Software User Guide. Wavelength optimization. . -Dual monochromatic optics -Wide range of applications -Top or bottom read modes The SpectraMax M3 Multi-Mode Microplate Reader provides three modes of detection in one platform The SpectraMax M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range. 7. SpectraMax fluorescence microplate reader with SoftMax® Pro Software, is a quick, sensitive detection method for proteins. 005) Photometric PrecisionThe Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. 4. Fluorescence intensity was measured using a. Twelve different automated tests are provided: • Fluorescence lower limit of detection (LLD) • Excitation wavelength accuracy • Emission wavelength accuracy • Excitation wavelength precisionHere, we present FluoroBrite™ DMEM, a cell culture media designed specifically for live-cell imaging that, when excited at wavelengths commonly used for FITC/GFP (i. ATP standard curves read on SpectraMax microplate readers. The Spectramax can accommodate cuvettes or a variety of 6 to 96-well plate formats. the IMAP Platform and smoothly transition into high-throughput screening. HTRF is a proprietary time-resolved fluorescence technology that overcomes The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. SpectraMax® Quant™ dsDNA Assay Kits The SpectraMax® Quant™ dsDNA Assay Kits are designed for fluorescence-based dsDNA quantitation across a broad range of concentrations. These settings are also valid for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port. The tunable SpectraMax M5e is a monochromator-based reader developed in collaboration with Cisbio international and is certified to run HTRF® assays. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. ATP concentration (M) Average RLU SpectraMax L SpectraMax i3x (cartridge) SpectraMax Mini SpectraMax iD5 SpectraMax i3x (onboard) SpectraMax M5e• SpectraMax® M2/M2e/M3/M4/M5/M5e Multi-Mode Microplate Readers • SpectraMax® Paradigm® Multi-Mode Microplate Reader • FlexStation® 3 Multi-Mode Microplate Reader • Gemini™ EM/XPS Fluorescence Microplate Readers • FilterMax™ F3/F5 Multi-Mode Microplate Readers Table 1. HEALTH AND SAFETY CONSIDERAIONSThe SpectraMax® L Microplate Reader measures flash and glow luminescence assays in both 96- and 384-well plates. . Examples: “Press carefully to engage,” “ Do not press down. For 40 years, we have partnered with scientists to help them achieve landmark discoveries. SpectraMax Plus, Plus384, M2, M2e, M5e or M5 readers). We would like to show you a description here but the site won’t allow us. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. UV/Vis Photometric Performance:. . . When used with SoftMax® Pro Data Acquisition and Analysis Software, it can automatically normalize the well absorbance to a cuvette equivalentAnalytical Technologies Group has deep experience maintaining and repairing Molecular Devices microplate readers, including the SpectraMax® M Series microplate readers (M2, M2e, M3, M5, M5, and M5e). It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. The SpectraMax M5e Microplate Reader enables five modes of detection with the additional benefit of being certified for Cisbio Bioassays’ HTRF Assays. . 2. The SpectraMax® M5 utilizes a single monochromator design for UV/VIS Absorbance, allowing the user to maximize sensitivity and tunability, and also uses patented PathCheck® Pathlength Measurement Technology: the only temperature independent pathlength correction for microplates. SpectraMax Plus, Plus384, M2, M2e, M5e or M5 readers). SpectraMax® M5/M5e Multimode Plate Reader, Molecular Devices Supplier: Molecular Devices Description: The SpectraMax® M5 Multi-Mode Microplate Reader delivers single. EUROPE. G03 through G06forSpectraMaxiD3,SpectraMaxiD5,SpectraMax i3x, SpectraMaxi3,M3,M4,M5,M5e,andFlexStation 3instruments G03 through G08forSpectraMaxLinstrument Cross-talk D09 through D11,E09 through E11,andF09 through F11 Linearity G03 through G06forSpectraMaxiD3,SpectraMaxiD5,SpectraMax. Catalog Number: M5. Molecular Devices SpectraMax M5 Multimode Microplate Reader. Microplate Readers include the M3, M4,. 72 MB. TR-FRET assay. Features. The SpectraMax M2 and M2e are versatile and reliable. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader. Data Acquisition & Analysis Software. Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. View the Molecular Devices website. Find many great new & used options and get the best deals for Molecular Devices SpectraMax M5 Microplate Reader with 90-Day Warranty at the best online prices at eBay! Free shipping for many products!Absorbance Detection Cartridges combine scanning and broad spectrum wavelength range to address a variety of nucleic acids, proteins, ELISAs and immunoassays found in the laboratory. Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. D 1. 3: The cuvette chamber. it is already turned on and the screen should look like this with “SpectraMax M5” showing as connected in the top left: 7. Molecular Devices SpectraMax M5 User Manual (86 pages) Multi-Mode Microplate Readers. Set the excitation at 531 nm and emission at 595 nm. . 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuidePlace the plate into a SpectraMax M5 e plate reader (Molecular Devices). The microplates were read the next day from the bottom and the top in SpectraMax M5 and Gemini EM readers. 304 signal level saturation. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. Description. 0 out of 5 $ 17,999 $ 16,995. Note: In this user guide, SpectraMax ® refers to several SpectraMax . 7°C (gradient heating design). Note: SoftMax Pro Software version 6 and 7 also include a searchable help file within the software. This user guide provides detailed instructions, troubleshooting tips and technical specifications for these instruments. The SpectraMax® iD3 Multi-Mode Microplate Reader and the SpectraMax® iD5 Multi-Mode Microplate Reader from Molecular Devices® have a touch screen interface that provides integrated instrument control, data display, and the ability to export results over your network for statistical data analysis. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. The introduction of the Molecular Devices’ SPECTRAmax PLUS microplate spectrophotometer marks the first time that photometric measurements made in a microplate format can be automatically normalized such that the resultant values are identical to those obtained with the corresponding solutions in a standard 1 cm pathlength cuvette. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. Molecular Devices SpectraMax M5/M5e Microplate Reader was tested for compatibility with Life Technologies LanthaScreen® Terbium-based TR-FRET assays. An assay plate containing a 15-point standard curve was read on the ® M5 Microplate reader. SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112-0115 Rev. Cavicide (Warehouse, Cat # 79300360) 6. UV/Vis Photometric Performance:. Category 2 and 3 microplate readers use gradient heating as well. Molecular Devices SpectraMax 190 Microplate Absorbance Spectrophotometer. SpectraMax ® M5 Multi-Mode Microplate Reader Transcreener ® ADP. The SpectraMax® M5 utilizes a single monochromator design for UV/VIS Absorbance, allowing the user to maximize sensitivity and tunability and also uses patented PathCheck® Pathlength Measurement Technology – the only temperature independent pathlength correction for microplates. Molecular Devices Spectramax M5 luminometer: Krishnamurthy lab-Praveen (lab WO3213, x4605) Spectramax plate reader: yes Beckman LS6000IC Scintillation counter: Bill Taylor (office WO4262B, x1966) WO3242 liquid scintillation counter Department backup -80oC freezer: Song-Tao Liu (WO4254, x7857) Facilities Facility: Department. 7°C (gradient heating design). The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. Cavicide (Warehouse, Cat # 79300360) 6. If you do not have an iLab account you must first make one: Calcium Assay Kits are the platform of choice for measuring changes of intracellular calcium for drug discovery and basic research. The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplates. ORDER. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. Certified SpectraMax® M5e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays’ HTRF (Homogeneous Time-Resolved Fluorescence) technology. M5 SpectraMax M5e Absorbance FluorescenceIntensity Luminescence Time-Resolved Fluorescence FluorescencePolarization ReadModes. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideThe SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays’ HTRF (Homogeneous Time-Resolved Fluorescence) technology. For luminescence, the SpectraMax M5 Microplate Reader utilizes a dedicated luminescence photomultiplier tube (PMT), providing the user the maximum signal and lowest background possible for glow luminescence reporter gene assays. The SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. Table of Contents. Softmax Pro GxP Software 6. When the correct COM port is selected, the. Products : SoftMax Pro GxP Software, SoftMax Pro Software, SpectraMax M5/M5e reader. 2 or higher, standard edition only, no GxP) VersaMax™ Microplate Reader (Serial numbers begining with BNxxxxx and BNRxxxxx are supported) SpectraMax® 190 Absorbance Microplate Reader (Serial numbers. Description 1. Recommended Optics Wavelength (nm) Wavelength selection Excitation 405/9 Monochromator Emission 1 450/15 Monochromator Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. INTRODUCTION The SpectraMax M2 and SpectraMax M2 e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as well SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers Operator’s Manual — 0112-0115 Rev. Spectranet customer portalMice were fasted overnight and orally administered FITC-dextran (600 mg/kg). The SpectraMax® M5 utilizes a single monochromator design for UV/VIS Absorbance, allowing the user to maximize sensitivity and tunability, and also uses patented PathCheck® Pathlength Measurement Technology:. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. Recertification is recommended every two years. . fluorescence polarization, trf, fret, igg quantitation, elisa, western blot, spectramax i3x, spectramax id5 multi-mode microplate reader, spectramax id5, spectramax paradigm, spectramax m5, spectramax m5 multi-mode microplate reader, high-throughput igg quantitation, mab igg titer, softmax pro, valita titer, valitatiter Created DateThe Spectramax M5 has similar detection capability to the Analyst GT and LMax II 384 (Figure 4), so we are confident that its performance is also similar when measuring the enzymes in cell lysates. The SpectraMax M5e Micoplate Reader is the standard for UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-well and 384-well microplates. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. D 1 1. Graph standard curves and calculate sample protein concentrations easily with a preconfigured. SoftMax Pro software can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. 72 MB. The dual monochromator optics allow. The SpectraMax M5 is a microplate reader that can be used for various applications, such as ELISA, Western blot, or cell culture. es • Flexibility for your assay needs: Robotics compatibility for increased throughput:In this application note, we describe how the SpectraMax® i3x Multi-Mode Microplate Reader with SpectraMax Injector Module is used to perform calcium assays on two different cell lines. Samples were measured together with a corresponding reference solvent contained in a. In this product video, see how the SpectraMax M5 quickly reads samples. Choose from a three- (M3), four- (M4), or SpectraMaxM3,M4,M5,andM5eMicroplateReaderUserGuide 26 0112-0115J ApplicationsofAbsorbance Absorbance-baseddetectioniscommonlyusedtoevaluatechangesincolororturbidity,. The KeySpan USA-19HS is often required with new Windows 7. Set up template in SoftMax Pro and read plate on the SpectraMax M5 microplate reader: Note: IMAP FP protocols for use with FAM- and TAMRA-labeled substrates are available in SoftMax Pro 5 in the Binding Assays protocol folder. Add to Cart. すべてのSpectraMax® Mシリーズマルチモードマイクロプレートリーダーで、マイクロプレートおよびキュベットを使用して、紫外光・可視光吸光度ならびに蛍光強度を測定できます。. BRET does not work on the Spectramax M5 due to a sensitivity issue and to the fact that the BRET assay has very weak signal in general. The guide also covers the software features, the specifications, and the accessories of the systems. Flexible for 6-well to 384-well microplates. 2. 1. Table of Contents. 2. I have been using the SpectraMax M5 since 2011. With optimized reagents, option to operate the readers. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. 3: The cuvette chamber. edu) to be added as a trained user in iLab. The drawing shows a standard microplate of 15 mm height covered with a lid. The Gemini XPS Reader has dual monochromators that allow users to utilize new and novel dyes without purchasing expensive filter sets. We would like to show you a description here but the site won’t allow us. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. Select a rugged, lightweight model designed to be used in the field. Wypalls Paper Towel (Warehouse, Cat # 79300335 or equivalent) 7. Step 1: Open the SoftMax Pro IMAP FP protocol. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. The following document is intended to demonstrate setup of this. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). Molecular Devices SpectraMax 384 Plus Microplate Reader Specifications. The SpectraMax ® i3x Platform from Molecular Devices is a multi-mode microplate reader that evolves with your future needs and offers an unlimited breadth of application possibilities. : 915 515 403 • Fax: 914 334 545 • e-mail: info@bionova. iD3, iD5, i3, i3x, M2, M2e, M3, M4, M5, M5e, FlexStation 3, Gemini EM, and Gemini XPS readers. For more sample throughput, the system canMolecular Devices SpectraMax Plus 384 UV/Vis Microplate Reader. Molecular Devices SpectraMax M5 Multi-mode Plate Reader 6. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. Setup recommendations for SpectraMax® M5e. HTRF is a proprietary time-resolved fluoresce nce technology that overcomes The SpectraMax i3x reader has variable bandwidth for fluorescence and luminescence, and you are able to choose either 9nm or 15 nm for excitation and either 15 nm or 25 nm for emission. SpectraMax L Microplate Reader provides medium to high throughput. Molecular Devices SpectraMax M5 Plate Reader available for sale at The Lab World Group. Neutrophils from six HC patients were stimulated by PMA with or without pretreatment of SIRL-1 mAb, and then co-cultured with autologous monocytes for 12 h. The optical performance is comparable to a top-of-the-line dedicated The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and drug discovery screening. . Category 2 and 3 microplate readers use gradient heating as well. 5039228D December2018 SpectraMax® i3x Multi-ModeDetectionPlatform WiththeSpectraMax® MiniMax™300ImagingCytometer UserGuideThe Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies Omnia® Assays. . For SpectraMax L or LMax II, LMax II. Issue: The audit trail in a SoftMax Pro GxP Software data file records a user as logging out of the system when the Auto Log Off prompt. The viscosity solution was prepared by mixing PBS and Glycerol in different proportion. 4. The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). These robust readers have been placed in labs from. Wavelength Range. spr SpectraMax iD3 SpectraTest ABS2 v1. The chamber contains springs that position the cuvette in the proper alignment for a read. The SpectraMax® M3, M4, M5 and M5 Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. SpectraMax i3x reader measures absorbance, fluorescence, & luminescence with user-upgradeable application including western blot imaging, AlphaScreen, fast kinetics along with injectors plus additional detection modes. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. To set up how to read the plate you can load an existing protocol from the protocol manager/protocol library or use the plate setup helper shown at the cursor below: 8. Fluorescent Polarization: Solid black plates, as above. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. The SpectraMax M2 and M2e systems provide the multi detection microplate readers with dualmonochromators, dual-mode cuvette ports, with top & bottom reading. Molecular Devices SpectraMax M5/M5e Microplate Reader was tested for compatibility with Life Technologies LanthaScreen® Terbium-based TR-FRET assays. For example, microplates containing different samples can be read using theLocate the User Guide on your SoftMax Pro computer for version 6 and version 7. The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. Molecular Devices ®(MD) SpectraMax 340PC reader MD VersaMax™ Tunable Microplate readers MD SpectraMax® L Microplate Luminometers MD SpectraMax® M5 systems MD ®SpectraMax 96/384-well plate reader Gemini fluorescence plate reader Automation Hamilton Microlab® STAR™ liquid handling systemThe SpectraMax® 190 spectrophotometer is ideal for most life science applications, especially DNA analysis. you can use a resource. Overall, SpectraMax M5 is a powerful lab tool that combines advanced detection capabilities, user-friendly software, and comprehensive accessory options. 2. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. While they play an important role in generating data from a wide range of. , ~488 nm), has the optical. free tracer decreases, resulting in an. The ability to measure inhibition of phosphorylation by Erlotinib was tested by incubating cells with increasing concentrations of Erlotinib, then stimulating cells with 0. . . Sale. It may be installed in the top or bottom read detection cartridge transport. DOCUMENTATION. 2. 306 plate air cal fail,low light. The system also gives users the flexibility to choose wavelengths between 200 nm and 1000 nm. A preconfiguredAcme Revival: Long Live DevicesWe would like to show you a description here but the site won’t allow us. The SpectraMax M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. By measuring optical pathlength, it eliminates the last major distinction between microplate readers and spectrophotometers. . . Monday to Friday 7am - 5pm Pacific Time. Some luminescence applications, such as gene reporter assays, may require a luminometer with photon counting detection for greater sensitivity, such as the SpectraMax M5, Analyst GT or Analyst HT, or LMax II. Description. ” SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Optimizing Fluorescence Polarization Assays Fluorescence polarization for SpectraMax M5 and M5 readers may only be read from the top of a microplate. SpectraMax M5 microplate reader. Cytokines in medium released. STAKMAX. The M5 can read volumes down to 2uL with the uMax Low Volume. I have been using the SpectraMax M5 since 2011. 7" color, capacitive touch screen. iD3, iD5, i3, i3x, M2, M2e, M3, M4, M5, M5e, FlexStation 3, Gemini EM, and Gemini XPS readers. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and found it an excellent resource for his lab's research on mTOR signaling in CD8 T cells. Methods Preparation of reagents and standards. view more . This analysis indicated that, after 48 hours incubation, there was a significant difference (P < 0. Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader This page contains the table of contents of resources available for the SpectraMax Microplate Reader Suite of Products. 5. 多功能酶标仪 FlexStation3 快速动力学操. What can be done for Communication problems with new Windows 7 computer and SoftMax Pro 5. The SpectraMax M5 utilizes a single monochromator design for UV/VIS Absorbance, allowing the user to maximize sensitivity and tunability, and also uses patented PathCheck Pathlength Measurement Technology: the only temperature independent pathlength correction for microplates. The SpectraMax ® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. The SpectraMax M5 Microplate Reader uses SoftMax® Pro. Fluorescence spectra and UV–vis spectra were recorded on a SpectraMax ® M5 multi-mode microplate reader (Molecular Devices). These instruments often feature additional detection modes in addition to fluorescence intensity, such as UV-vis absorbance, fluorescence polarization, and time-resolved.